Fig. 7.

Delayed chloroplast division in the absence of F-actin. Chlorophyll autofluorescence and EB1-mNG fluorescence (to visualize the ingressing cleavage furrow) are shown in a nap1-1 cell not treated with LatB (A) and a nap1-1 cell treated with 3 µM LatB beginning ∼20 min before the first frame shown (B). Cells had been synchronized prior to imaging using the 12L:12D/TAP agar method. Arrowheads, times of apparent completion of chloroplast division. (Scale bars, 5 µm.)