Fig. 5.

Coexpression of antagonistic RCD-1–1 and RCD-1–2 variants triggers pyroptotic-like cell death in human 293T kidney cells. (A) Images of human 293T cells transfected with plasmids expressing mCherry- or eGFP-tagged RCD-1–1 or RCD1-2 in compatible combinations (RCD-1–1 + RCD-1–1 or RCD-1–2 + RCD-1–2) or antagonistic combinations (RCD-1–1 + RCD-1–2). Representative pyroptotic-like cells are indicated with an arrowhead. (Scale bar, 100 µm.) (B) Quantification of mCherry and eGFP double-positive cells for compatible and antagonistic RCD-1 combinations. Values are relative to cells transfected with mCherry-RCD-1–1 + eGFP-RCD-1–1. Welch’s unpaired t test *P < 0.01, **P < 0.001. ns, nonsignificant.