Fig. 6.

Protein–protein interactions between antagonistic RCD-1 allelic variants in human 293T cells. co-IP experiments with HA- and FLAG-labeled RCD-1–1 and RCD-1–2 variants. Numbers indicate the amount of DNA vector (nanograms) used for each transfection reaction. Dashes indicate that the vector has not been used in a transfection reaction. Lysates are defined as input, where the presence of the HA-labeled RCD-1 variants (top blot) and the FLAG-labeled (second blot) RCD-1 variants are shown. The immunoprecipitation of HA-RCD-1–1 and HA-RCD-1–2 pulls down both FLAG-tagged RCD-1 variants (IP:HA blots).