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. 2020 Aug 7;11:3953. doi: 10.1038/s41467-020-17740-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a UMAP visualization of the mural cell dataset, color coded and annotated for pagoda2 clustering (left) or color coded for organ of origin and annotated for pagoda2 clustering (right). b Trajectory analysis plots of the mural dataset color coded for pagoda2 clusters (left) or organ of origin (right). c Expression heat map (black, low; yellow, high) of cluster enriched genes (see also Online Supplementary Data 9). d Samples from all four organs from the Pdgfrb^GFP reporter line, stained with immunofluorescence for αSMA and PECAM1. Arrows highlight pericytes and their morphological distribution at the organ-specific capillaries. Scale bars: 50 µm. e Immunofluorescence staining of colon samples from Pdgfrb^GFP reporter line for NG2 (Cspg4) and PECAM1 (left panel), or αSMA, CNN1 and PECAM1 (right panel). f Immunofluorescence staining of colon samples from Cspg4^dsRED reporter line for PDGFRβ, CNN1 and PECAM1. Arrows highlight colon pericytes located at the subepithelial capillary loop, arrowheads indicate αSMA⁺ CNN1⁺, but Cspg4 (NG2)⁻ interstitial SMC. Scale bars: e 100 µm (left panel f), 50 µm (e right panel).