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. 2020 Aug 7;11:3940. doi: 10.1038/s41467-020-17858-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Heat map of the intensity of i-BLESS signal at TTS in control and shTop1 cells for two groups of genes determined according to the intensity of i-BLESS signal at the TTS (±2 kb) in shTop1 cells (Top 25%). b Metaplots of i-BLESS, RNA:DNA hybrids and p-RPA32 S33 signal for the Top 25% (red) and other (black) genes in control and shTop1 HeLa cells. Error bars indicate SEM. Differences in signal intensity at TTS ±2 kb were calculated with the Wilcoxon rank-sum test with continuity correction.