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. 2020 Aug 7;10:13352. doi: 10.1038/s41598-020-70392-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of Bay 11-7082 and TMZ on signaling pathways in patient-derived GBM cells. (a) Heat map presentation of RPPA analysis showing the changes in the protein expression. RPPA was performed on lysates treated with Bay 11-7082 and TMZ alone or in combination. All relative protein level data points were normalized to the control group. Red and green indicate up and down regulations, respectively in the heat map. The samples were run in duplicate (n = 3). (b) Fold change of the selected proteins relative to the control group via RPPA. Data represent the mean ± SD of two biological replicates. (* p < 0.05, ** p < 0.01, Wilcoxon rank sum test). (c) Analysis of down-regulated RPPA proteins shows a significant activation in numerous Enrichr KEGG pathways. The pathways were sorted by p value ranking. (d) Representative immunoblot validation of significantly altered proteins involved in different KEGG pathways. Patient-derived GBM cells were cultured with or without drugs for 7 days, lysed and immunoblotted with the indicated antibodies. Quantification of the fold-changes in protein levels (right panel). Data were normalized to B-actin. Data represent the mean ± SD of three biological replicates. * p < 0.05, ** p < 0.01.