Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 21;117(31):18764–18770. doi: 10.1073/pnas.2002651117

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Published under the PNAS license.

PMC Copyright notice

Fig. 4. — FOXO3a directly binds to FOXO consensus sites in iP2. (A) Recombinant purified FOXO3a was incubated with double-stranded biotinylated probes containing the indicated FOXO consensus sites in iP2 (site 1, site 2, and site 3), or mutated sequences containing the changes shown in Fig. 1 (site 1 mut, site 2 mut, and site 3 mut). Protein:DNA complexes were resolved on nondenaturing acrylamide gels, and the complexes were visualized by chemiluminescence. The data are representative of three independent experiments. (B) Graph showing the decrease in FOXO3a binding to the mutant probes compared to the wild-type probes, which are set to 100%. The graph shows the mean of three independent experiments (*P < 0.05; ***P < 0.0005).