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. 2019 Jul 15;100(9):1273–1281. doi: 10.1099/jgv.0.001294

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© 2019 The Authors

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Fig. 2. — RNP polymerase activity was higher in the presence of PB2-292V than PB2-292I in human 293T cells. RNP polymerase activities of wild-type and mutant viruses in (a) 293T and (b) avian DF1 cells, transfected with polymerase plasmids (PB1, PA, NP and PB2-292I or PB2-292V), were determined by mini genome replication assays at different temperatures with reference PB2-292I at 100 %. Data are presented as means±sd of three independent experiments. Statistical significance was based on two-way ANOVA (*P<0.05, **P<0.01, ***P<0.001).