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. 2019 Jul 15;100(9):1273–1281. doi: 10.1099/jgv.0.001294

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© 2019 The Authors

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Fig. 3. — Avian H9N2 virus with PB2-292V generated more mRNA and vRNA of viral M1 and NP genes than the same virus with PB2-292I. A549 cells were infected separately with H9N2:PB2-292I and H9N2:PB2-292V at 0.2 m.o.i. for 2, 6, 12 and 24 h. Normalized mRNA and vRNA levels of M1 and NP at each time point are expressed as fold-change relative to H9N2:PB2-292I. Data are presented as means±sd of three independent experiments. Statistical significance was based on two-way ANOVA (*P<0.05, **P<0.01, ***P<0.001).