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Avian H9N2 virus with PB2-292V replicated more effectively than the same virus with PB2-292I in (a) A549 and (b) MDCK cells. Multistep growth curves of H9N2 viruses were determined in A549 and MDCK cells at an m.o.i. of 0.2 and 0.001, respectively. Virus titres were determined from supernatants collected at the indicated time points. Data are presented as means±sd of three independent experiments. Statistical significance was based on two-way ANOVA (*P<0.05, **P<0.01, ***P<0.001).
