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. 2019 Jul 15;100(9):1273–1281. doi: 10.1099/jgv.0.001294

Fig. 6.

Fig. 6.

PB2-I292V mutation in H9N2 virus raised pathogenicity and viral replication in mice. Groups of BALB/c mice were inoculated with 106 TCID50 of the indicated in mice. Groups of BALB/c mice were inoculated with 106 TCID50 of the indicated virus or mock infected with PBS. (a) Body weight changes of mice inoculated with H9N2:PB2-292I and H9N2:PB2-292V virus. Data are presented as means±sd of five individual mice. (b) Survival rates of mice. Mice infected with PB2-292V virus showed higher morbidity than PB2-292I virus. Mice with weight loss of more than 25 % were euthanized. (c) Hematoxylin and eosin (H&E) stained lung sections of mice infected with indicated virus at 5 days p.i. Scale bar, 200 μm. (d) Virus titres in lungs harvested from three mice per group at 3, 5 and 7 days p.i. Virus titres are means±sd of three individual mice. Statistical significance was based on two-way ANOVA (*P<0.05, **P<0.01, ***P<0.001).