Fig. 3.

Phylogenetic relationship of ASALV. The phylogenetic tree was inferred based on a MAFFT-E translational alignment of the nucleotide sequences of the region of the structural protein ORF encoding the E2, 6K and E1 protein of all established alphavirus species, TALV, MWAV and ASALV strain PA-2013-MP416. An optimized maximum-likelihood phylogenetic tree with the GTR substitution model and 1000 bootstrap replicates was calculated using PhyML. The tree was rooted to the midpoint. GenBank accession numbers are shown next to the virus names. EEE, eastern equine encephalitis; VEE, Venezuelan equine encephalitis; WEE, western equine encephalitis; SF, Semliki Forest.