Fig. 4.

HDACi drive MIEP-derived transcription in experimentally latent CD14+cells. Experimentally latent CD14+cells were incubated with PMA or the HDACi Romdepsin for 24 h and then RNA analysed by qRT-PCR with primers that amplified MIEP-derived IE transcripts (MIEP), ip2-derived UTR70 transcripts (UTR70) and cellular 18S gene expression 24 h post stimulation. Gene expression is reported relative to the RNA expression observed in the unstimulated (for PMA) or DMSO controls (for HDACi) using the 2delta deltaCT method from three independent experiments. *P<0.05; **P<0.01; ***P<0.001.