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ZIKV infectivity in hOEC and hCMEC/D3 cells. hOEC and hCMEC/D3 cells were infected with ZIKV at an m.o.i. of 1 (calculation based on titre from Vero cells). The cells were harvested at 16 h p.i. and ZIKV NS3 expression was measured by staining with anti-NS3 antibody and FACS analysis. At least 100 000 events were captured per sample. Each bar represents the mean±sem of two independent experiments performed in triplicate. *P<0.01.
