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. 2020 Apr 7;101(6):609–621. doi: 10.1099/jgv.0.001410

Table 2.

List and sequence relationships of various picornaviruses of four genera identified by NGS and blast analyses from a single faecal specimen of a 3-week-old paraplegic pig (SZ1M). Where it was necessary the missing genomic regions from the complete coding sequences (CDS) or complete genomes were determined by RT-PCR and Sanger sequencing methods using contig-specific primer pairs (data not shown). The lengths of the determined viral sequences are found in the ‘Final sequence length’ column

Genus

No. of reads

No. of contigs

Total length of the contig(s)

Final sequence length

Genome completeness

Accession No.

Closest relative by blast

nt identity

aa identity

Enterovirus

3205

4

5331 nt

7228 nt

complete CDS

MN807749

Porcine enterovirus 3 h [HQ702854]

78 %

88 %

Kobuvirus

895

1

7951 nt

7951 nt

complete CDS

MN807751

Porcine kobuvirus 1 [GQ249161]

89 %

96 %

Teschovirus

674

2

6593 nt

6981 nt

complete CDS

MN807750

Porcine teschovirus 3 [MF170908]

87 %

96 %

Sapelovirus

111

1

779 nt

7534 nt

complete genome

MN807752

Porcine sapelovirus 1 [LC425415]

78 %

84 %

nt, nucleotide; aa, amino acid.