Table 2.
List and sequence relationships of various picornaviruses of four genera identified by NGS and blast analyses from a single faecal specimen of a 3-week-old paraplegic pig (SZ1M). Where it was necessary the missing genomic regions from the complete coding sequences (CDS) or complete genomes were determined by RT-PCR and Sanger sequencing methods using contig-specific primer pairs (data not shown). The lengths of the determined viral sequences are found in the ‘Final sequence length’ column
|
Genus |
No. of reads |
No. of contigs |
Total length of the contig(s) |
Final sequence length |
Genome completeness |
Accession No. |
Closest relative by blast |
nt identity |
aa identity |
|---|---|---|---|---|---|---|---|---|---|
|
Enterovirus |
3205 |
4 |
5331 nt |
7228 nt |
complete CDS |
Porcine enterovirus 3 h [HQ702854] |
78 % |
88 % |
|
|
Kobuvirus |
895 |
1 |
7951 nt |
7951 nt |
complete CDS |
Porcine kobuvirus 1 [GQ249161] |
89 % |
96 % |
|
|
Teschovirus |
674 |
2 |
6593 nt |
6981 nt |
complete CDS |
Porcine teschovirus 3 [MF170908] |
87 % |
96 % |
|
|
Sapelovirus |
111 |
1 |
779 nt |
7534 nt |
complete genome |
Porcine sapelovirus 1 [LC425415] |
78 % |
84 % |
nt, nucleotide; aa, amino acid.