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. 2020 Jul 1;13(7):1590–1600.

Figure 2.

Figure 2

SMADs signaling regulates expression of tight junction protein and E-cadherin. Real-time polymerase chain reaction (RT-PCR) analysis of tight junction protein (ZO-2, ZO-1, occludin, CLDN7, and CLDN6) and E-cadherin; and densitometric analysis of relative gene expression levels after normalization to loading control GAPDH are presented. The lane “M” stands for marker; (*P < 0.05 and **P < 0.01 are considered significant and highly significant, respectively). Bars represent mean ± SE (n = 3).