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. 2020 Jul 1;13(7):1590–1600.

Figure 3.

Figure 3

CLDN6 is required for the breast cancer cell TEER, morphology, and clone formation. A, B. CLDN6-mediated inactivation of SMADs signaling pathway regulated TEER (*P < 0.05 and **P < 0.01 are considered significant and highly significant, respectively). bars represent mean ± SE (n = 3). C. RT-PCR and immunoblot expression analyses of CLDN6 in MCF-7-shGFP, MCF-7-shCLDN6, SKBR-3-shGFP, and SKBR-3-shCLDN6 cells pretreated by SB431542, and densitometric analysis of relative expression levels after normalization to loading control GAPDH or β-actin are presented. The lane “M” stands for marker (*P < 0.05 and **P < 0.01 are significant and highly significant, respectively). Bars represent mean ± SE (n = 3). D, E. CLDN6-mediated inactivation of SMADs signaling pathway affected cell morphology (Bar, 100 μm). F, G. CLDN6 was required to inhibit cell clone formation (*P < 0.05 and **P < 0.01 are significant and highly significant, respectively). Bars represent mean ± SE (n = 3).