Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 8;39:151. doi: 10.1186/s13046-020-01660-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — LINC00514 promoted PC cell proliferation, migration and invasion. (a-b) Transfection efficiency of LINC00514 overexpression plasmids and shRNA in BxPC-3 and SW1990 cells were evaluated by qRT-PCR. (c-d) CCK-8 assay was performed to detect cell (BxPC-3, SW1990) proliferation ability with LINC00514 overexpression and LINC00514 silencing. (e) Colony formation assay was carried out to further detect cell proliferation capacity. (f-g) Transwell migration and invasion assay were carried out to detect cell migration and invasion under LINC00514 overexpression and knockdown. (h) Western blot was conducted to evaluate the impact of LINC00514 on EMT progression. *p<.05, **p<.01, **p<.001. All experiments were repeated at least for three times and mean±SD was used to represent the final result. PC, pancreatic cancer; qRT-PCR, quantitative real-time polymerase chain reaction; SD, standard deviation; CCK-8, cell counting kit-8; EMT, epithelial-mesenchymal transition