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. 2020 Aug 8;39:151. doi: 10.1186/s13046-020-01660-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Rap1b was a downstream target of miR-28-5p in PC. (a) The sequence of WT-Rap1b, Mut-Rab1b and miR-28-5p were conducted. (e-f) Luciferase reporter assay and RIP assay were performed to determine the association between miR-28-5p and Rap1b. (f-g) QRT-PCR and western blot were used to detect Rap1b expression in cells of LINC00514 silencing and cells of co-transferring miR-28-5p and LINC00514 shRNA at transcription and translation level. (h) Relative Rap1b expression in tumor tissue and normal tissue were detected by qRT-PCR. (i-g) The correlation between Rap1b and LINC00514 as well as the correlation between Rap1b and miR-28-5p were analyzed by Spearman’s rank correlation test. (k-l) The Rab1b expression levels under LINC00514 silencing and LINC00514 overexpression were evaluated in vivo. (m-n) CCK-8 assay was performed to detect proliferation of cells transfected with miR-28-5p inhibitor and cells co-transfected with miR-28-5p inhibitor and Rap1b shRNA. (O-P) Transwell migration and invasion assay were carried out to detect cell migration and invasion abilities. *p<.05, **p<.01, **p<.001. All experiments were repeated at least for three times and mean±SD was used to represent the final result. PC, pancreatic cancer; qRT-PCR, quantitative real-time polymerase chain reaction; RIP, RNA immunoprecipitation; SD, standard deviation