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. 2020 Aug 8;11:346. doi: 10.1186/s13287-020-01856-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Isolation and identification of EnSCs. a Morphology of EnSCs. P0 and P3 EnSC-Control showed a typical spindle-shaped, fibroblast-like morphology with a radial or helical growth pattern, but EnSC-EM-EC showed an irregular morphology instead of a spindle-shaped, fibroblast-like morphology. Furthermore, the expression of vimentin in EnSC-Control and EnSC-EM-EC cells are examined by conventional immunofluorescence. Representative images are shown. b Phenotype of EnSCs. Both EnSC-Control and EnSC-EM-EC were positive for the expression of typical ASC markers (CD29, CD73, CD90 and CD105) and HLA-ABC and negative for the expression of haematopoietic stem cell markers (CD34 and CD45) and HLA-DR. c Conventional adipogenic and osteogenic differentiation were induced, and differentiation was visualized as positive Oil red O and Alizarin red staining. Scale bar, 25 μm