Fig. 2.

Proliferative and migratory capacities of EnSCs. a, b P3 EnSC-Control and EnSC-EM-EC were used to assess the cell cycle status as directed by the kit instruction. Representative flow cytometric results are shown. c Quantification of flow cytometric cell cycle assay results. d P3 EnSC-Control and EnSC-EM-EC were seeded into 96-well plates, and the proliferative capacity was determined by a conventional MTT assay at the indicated time points. e P3 EnSC-Control and EnSC-EM-EC cells were seeded into 6-well plates and cultured to 100% confluence. Then, wound healing assay was performed to examine the number of cells migrating into the wounded area at the indicated time points. Representative images are shown. Scale bar, 50 μm. f Quantification of the wound healing assay results. g, h P3 EnSC-Control and EnSC-EM-EC in serum-free culture medium were seeded in the upper chamber of inserts in 24-well plates and were allowed to migrate towards growth medium supplemented with 10% FBS for 24 h. Then, the cells that migrated to the lower surface of the insert were fixed and stained with crystal violet. Representative images are shown. i Quantification of the transwell assay results