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Quantification. 12 points dilution curves were run for CR3022_IgG (A), CR3022_IgA (B) and CR3022_IgM (C) monoclonal. The dotted lines show linear range between ULOQ and LLOQ. A five-parameter log-logistic function (Y = c + (d - c)/((1 + exp.(b*(log(X) - log(e))))^f)) allows the conversion of OD in concentration of antibody (μg/ml).
