Fig. 1. Characterization of droplet interface bilayers.

a Schematic representation of the ER phospholipid bilayer contiguous with the monolayer of a nascent LD (left side); the corresponding DIB system reproducing contiguous bilayer and monolayers is shown on the right side. The water phase is represented in light blue and the oil phase in yellow (neutral lipid, e.g. triglycerides (TG)). b Drawings of a DIB bilayer of DOPE (top) and DOPC/DOPE (1:1) (bottom). c The thickness of the hydrophobic region of the DIB bilayer in DOPE (white) and DOPC/DOPE (1:1) (gray) is determined by capacitance measurement. Results are shown as box-plots (box limits, upper and lower quartiles; middle line, median; whiskers, minimum and maximum value; the mean is indicated) from n = 5 independent experiments. Each point is represented as a black dot. d Distribution of Rh-PE between the bilayer and the monolayers in DOPE (white) and DOPC/DOPE (1:1) (gray) DIBs. The results are the mean ± SD of respectively n = 10 and n = 5 independent measurements. Each point is represented as a black dot. Significance was determined by Welch’s t-test (unpaired parametric test, two-tailed p-value) and is indicated by ns (not significant): p > 0.05. Source data are provided as a Source Data file.