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. 2020 Aug 7;10:13311. doi: 10.1038/s41598-020-69327-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Manual assessment of expression of key markers following Cb vaccination and challenge. Manually gated populations were used to determine the frequency of parent for Ly6C+, T-bet+, or CD73+ cells in major immune populations from tgHLA-DR3 mice. Heatmaps illustrate the median frequency of parent within each group of mice (n = 8) on Days 10 and 35 post-vaccination, and Days 2 and 10 post- challenge (study Days 44 and 51). The color-coded scale reflects the frequency of parent for each heatmap. The Kruskal–Wallis test for non-parametric data was used to identify statistically significant changes based on pairwise comparisons between treatment groups within each timepoint; the p-value for significant changes are indicated on the graph.