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. 2020 Aug 7;11:3962. doi: 10.1038/s41467-020-17771-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Putative second order neurons of UNs (red) revealed by trans-Tango (green). Magenta: nc82. Right panel: the abdominal ganglion (Abg) of the VNC was zoomed in. White dashed line outlines the border of the VNC. Scale bar represented 50 μm. b Immunofluorescence of abdominal LK neurons (ABLK) (green) and nc82 (magenta). Scale bar represented 50 μm. c GRASP signal (green) between UNs and LK neurons in the Abg. White dashed line outlines the border of the VNC. Scale bar represented 50 μm. d, e Mutation of the LK gene d or silencing LK neurons with Kir2.1 e blocked the mating-induced defense facilitation. n = 9, 8, 12, 11, 9, 9 (from left to right) in d, n = 10, 10, 8, 8, 9, 8 (from left to right) in e. Error bars indicate mean ± SEM, **p < 0.01, *p < 0.05, p > 0.05 (n.s.), n.s., not significant, two-tailed unpaired t tests. Source data are provided as a Source Data file.