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. 2020 Aug 7;10:13342. doi: 10.1038/s41598-020-70165-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Biochemical analysis. Expression levels of cytochrome c have been assessed in thyroids of five patients; for each patient, the healthy (Hea) and pathological (TIR) slices have been analysed. (A) Levels of cytochrome c have been normalized to actin. Data represent the mean values ± SDs derived from three replicates normalized to healthy counterparts (Student’s t test, *P < 0.05, **P < 0.01 compared with control). (B) Exemplificative images of filters blotted with cytochrome c and actin primary antibodies. Images have been gathered at the same time.