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. 2020 Aug 7;10:13329. doi: 10.1038/s41598-020-70277-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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shNOTCH2 cells exhibited less ALP enzymatic activity and mineralization in Jagged1 treated condition. hPDLs were seeded on Jagged1-immobilized surfaces. The control condition was hFc-immobilized surfaces. Mineral deposition was examined using alizarin red s staining at day 14 (A). Absorbance of the solubilized dye (B). ALP staining and enzymatic activity assay were evaluated at day 3 and 7 after osteogenic differentiation (C–E). An in vitro mineralization was stained with alizarin red s dye (F). Absorbance of the solubilized dye (G). Bars indicate a significant difference. GM growth medium, OM osteogenic medium.