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. 2020 Aug 7;10:13332. doi: 10.1038/s41598-020-70258-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licens»e, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Electrotransfection efficiency of MEF and iPS cells. In each electroporation reaction, 20 µg (1.5–2.5 µg/µl) of either pT2-Venus and pT2-mCherry which encode Venus and mCherry proteins, respectively, were pre-mixed with cells and underwent electroporation. The following electroporation program was used: square-wave protocol with either 250 V for iPS or 300 V for MEF cells, each 10 ms pulse length, 2 pulses, 10 s pulse interval, and 4 mm cuvette. (a) Transfection efficiency. Transfected cells for mCherry and Venus are depicted by red and green bars, respectively. (b) Expression of Venus and mCherry 36 h after electroporation. Scale bar for iPS cells equals 100 µm. Scale Bar in fibroblasts is 10 µm. Results are means and standard deviation (n > 3).