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. 2020 Aug 4;12:6849–6860. doi: 10.2147/CMAR.S253510

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© 2020 Fang et al.

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MiR-1258 directly targeted RPN2 in HCC. (A) QRT-PCR results indicated that the mRNA expression of RPN2 was increased in HCC tissues (HCC) compared with adjacent normal tissues (Normal). WB analysis results suggested that the protein expression levels of RPN2 were higher in HCC tissues (B) and cells (C) than that in matched controls. Pearson correlation coefficient analysis was employed to analyze the correlation between RPN2 and circ_0046599 (D) or miR-1258 (E). (F) The binding sites and mutant binding sites between RPN2 and miR-1258 were shown. Dual-luciferase reporter assay (G) and RIP assay (H) were performed to confirm the interaction between RPN2 and miR-1258. (I) The RPN2 protein expression in HCCLM3 and Huh cells was measured by WB analysis to assess the effect of miR-1258 and circ_0046599 expression on RPN2 expression. ***P < 0.001.