TABLE 1.
Predicted T9SS type A CTD-containing proteins of F. psychrophilum strain OSU THCO2-90a
| Locus tag | Protein | Length (aa)b | Molecular mass (kDa)c | Domain(s)d | Description/known or predicted function |
|---|---|---|---|---|---|
| THC0290_0086 | 642 | 70.9 | M1_APN_like (cd09603), peptidase_M1 (pfam01433) | Probable peptidase precursor (M1 family metalloprotease) | |
| THC0290_0091 | 1,139 | 122.8 | Sortilin-Vps10 superfamily (cl25791), peptidase_M6 superfamily (cl29544) | Probable glycoside hydrolase precursor | |
| THC0290_0128 | 113 | 12.8 | |||
| THC0290_0129 | 324 | 33.6 | Putative outer membrane protein precursor | ||
| THC0290_0170 | 398 | 41.7 | LRR_5 (pfam13306) | Probable cell surface protein (leucine-rich repeat protein) precursor | |
| THC0290_0171 | 421 | 44.5 | LRR_5 (pfam13306) | Probable cell surface protein precursor (leucine-rich repeat protein) precursor | |
| THC0290_0172 | 329 | 35.2 | LRR_5 (pfam13306) | Probable cell surface protein (leucine-rich repeat protein) | |
| THC0290_0173 | 444 | 47.2 | LRR_5 (pfam13306) | Probable cell surface protein (leucine-rich repeat protein) precursor | |
| THC0290_0174 | 422 | 44.5 | LRR_5 (pfam13306) | Probable cell surface protein (leucine-rich repeat protein) precursor | |
| THC0290_0175 | 330 | 35.3 | LRR_5 (pfam13306) | Probable cell surface protein (leucine-rich repeat protein) precursor | |
| THC0290_0176 | 375 | 40.1 | LRR_5 (pfam13306) | Probable cell surface protein (leucine-rich repeat protein) precursor | |
| THC0290_0177 | 536 | 56.0 | LRR_5 (pfam13306) | Probable cell surface protein (leucine-rich repeat protein) precursor | |
| THC0290_0178 | 307 | 33.1 | LRR_5 (pfam13306) | Probable cell surface protein (leucine-rich repeat protein) precursor | |
| THC0290_0179 | 421 | 44.3 | LRR_5 (pfam13306) | Probable cell surface protein (leucine-rich repeat protein) precursor | |
| THC0290_0180 | 398 | 42.1 | LRR_5 (pfam13306) | Probable cell surface protein (leucine-rich repeat protein) precursor | |
| THC0290_0181 | 467 | 50.1 | LRR_5 (pfam13306) | Probable cell surface protein (leucine-rich repeat protein) precursor | |
| THC0290_0182 | 329 | 35.0 | LRR_5 (pfam13306) | Probable cell surface protein (leucine-rich repeat protein) precursor | |
| THC0290_0183 | 306 | 33.2 | LRR_5 (pfam13306) | Probable cell surface protein (leucine-rich repeat protein) precursor | |
| THC0290_0184 | 351 | 37.0 | LRR_5 (pfam13306) | Probable cell surface protein (leucine-rich repeat protein) precursor | |
| THC0290_0185 | 329 | 35.3 | LRR_5 (pfam13306) | Probable cell surface protein (leucine-rich repeat protein) precursor | |
| THC0290_0186 | 328 | 35.3 | LRR_5 (pfam13306) | Probable cell surface protein (leucine-rich repeat protein) precursor | |
| THC0290_0237 | Fpp1 | 1,138 | 119.6 | ZnMc superfamily (cl00064), FN3 superfamily (cl21522), P_proprotein superfamily (cl27557) | Peptidase precursor (psychrophilic metalloprotease Fpp1) |
| THC0290_0238 | Fpp2 | 942 | 100.3 | ZnMc_pappalysin_like (cd04275), cleaved_adhesin superfamily (cl06636) | Peptidase precursor (psychrophilic metalloprotease Fpp2) |
| THC0290_0299 | 914 | 100.1 | Peptidase_M36 (pfam02128), PA_subtilisin_1 (cd04818) | Probable peptidase precursor (M36 fungalysin family metalloprotease) | |
| THC0290_0300 | 919 | 99.8 | Peptidase_M36 (pfam02128), PA_subtilisin_1 (cd04818) | Probable peptidase precursor (M36 fungalysin family metalloprotease) | |
| THC0290_0878 | 747 | 78.2 | FN3 (cd00063) | Putative adhesin precursor | |
| THC0290_0931 | 959 | 103.2 | ZnMc superfamily (cl00064) | Peptidase precursor (collagenase; Zn-dependent metalloprotease) | |
| THC0290_0944 | 544 | 58.5 | Peptidases_S8_9 (cd07493) | Probable peptidase precursor (S8 subtilisin family serine endopeptidase) | |
| THC0290_0977 | 585 | 64.9 | Abhydrolase superfamily (cl21494) | Esterase/lipase/thioesterase family protein precursor | |
| THC0290_1054 | 408 | 46.0 | SGNH_hydrolase superfamily (cl01053), PKD repeat (COG3291) | ||
| THC0290_1343 | 1,218 | 134.1 | ZnMc superfamily (cl00064), PKD (COG3291, 3 copies), PCC superfamily (cl28216) | Probable cell surface protein precursor; putative metallopeptidase, metzincin clan | |
| THC0290_1494 | 657 | 71.5 | HNHc superfamily (cl00083) | Probable ribonuclease | |
| THC0290_1520 | 613 | 66.2 | Endonuclease_1 (pfam04231), FN3 (cd00063), COG2374 superfamily (cl28586) | Probable endonuclease precursor | |
| THC0290_1595 | 628 | 67.9 | Putative adhesin precursor | ||
| THC0290_1797 | 263 | 29.4 | DOMON_DOH (cd09631) | ||
| THC0290_2029 | 301 | 32.9 | |||
| THC0290_2146 | PorU | 1,278 | 142.1 | Peptidase_C25_N (cd02258) | Peptidase precursor (T9SS CTD signal peptidase, PorU) |
| THC0290_2157 | 166 | 18.7 | |||
| THC0290_2385 | 456 | 49.2 | ATS1 superfamily (cl34932), RCC1 (pfam00415) | RCC1 (regulator of chromosome condensation) repeat domain protein precursor | |
| Avg | 550 | 59.3 | |||
| Median | 422 | 46.0 | |||
| Range | 113 to 1,278 | 12.8 to 142.1 | |||
a
The T9SS type A C-terminal domain (CTD) (TIGR04183) is a C-terminal domain that targets a protein for secretion by the T9SS. TIGR04183 is described as “Por secretion system C-terminal sorting domain” on the J. Craig Venter Institute TIGR website (http://www.jcvi.org/cgi-bin/tigrfams/index.cgi). Each of these proteins contains an N-terminal signal peptide for export across the cytoplasmic membrane and a T9SS type A CTD for secretion across the outer membrane by the T9SS, as determined using SignalP 5.0 with a cutoff of 0.8 and the Integrated Microbial Genomes (IMG version 4.0.1 [https://img.jgi.doe.gov/]) tools and trusted cutoffs, respectively.
b
Length in amino acids (aa) of full-length protein before removal of signal peptide or CTD.
c
Molecular mass (kDa) of amino acids in full-length protein before removal of signal peptide or CTD.
d
Domains other than signal peptide and CTDs, with pfam, cl, cd, or COG numbers indicated.