FIG 4.

Secreted lipase scanning in S. albus ZD11. (A) Phylogenetic relationship of the secreted lipases from the four Streptomyces strains. Gray circles represent the lipases from S. albus ZD11. Diameters of the light purple bubbles on the branches represent the bootstrap values of the N-J tree. Gene IDs with the prefixes SCO, XNR, and SLIV represent lipase genes from S. coelicolor A3(2), S. albidoflavus J1074, and S. lividans TK24, respectively. (B) Determination of total lipolytic activity. Diameters of the fluorescent halos under UV represent lipolytic activities. (C) Quantification of the total lipolytic activity in the oil medium containing 2.7 M carbon. (D) Expression level analysis of secreted-lipase genes based on RNA-seq and RT-qPCR data. Error bars indicate the SD of samples performed in triplicate.