FIG 5.

Multiple lipases work in a synergistic way to support efficient and stable TAG hydrolysis in S. albus ZD11. (A) Total lipolytic activity, as determined by the RhB plate assay. (B) Comparison of total lipolytic activities between the Δlip1 Δlip2 mutant and WT strain in a liquid fermentation time course. (C) Expression level analysis of the remaining lipase genes in the Δlip1 Δlip2 mutant. RNA samples were isolated from 60-h cultures. hrdB transcription was monitored and used as the internal control. Values are relative to those of the WT strain. Error bars indicate the SD for samples tested in triplicate.