FIG 6.

Determination of the hydrolytic activity of Lip1. (A) SDS-PAGE of purified truncated Lip1 with a double 6×His tag located at both ends of the peptide. Lane 1, 1.0 μg protein; lane 2, 0.8 μg protein; lane 3, 0.5 μg protein. (B through D) Effects of temperature (B), pH (C), and substrate (D) on the activity of Lip1. C4, C8, C12, and C18 represent tributyrin, tricaprylin, trilaurin, and triolein, respectively. (E) Kinetic curve for Lip1 activity. Error bars indicate the SD for samples tested in triplicate.