TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| S. albus ZD11 | Derivative obtained by the streak plate method from an industrial salinomycin-producing strain | CGMCC 4.7658 |
| S. albus ZD11 Δlip1 | ZD11 with disruption of lip1; Spr | This study |
| S. albus ZD11 Δlip2 | ZD11 with disruption of lip2; Spr | This study |
| S. albus ZD11 Δlip1/plip1 | Δlip1 harboring plip1 | This study |
| S. albus ZD11 Δlip2/plip2 | Δlip2 harboring plip2 | This study |
| S. albus ZD11 Δlip1 Δlip2 | ZD11 with disruptions of lip1 and lip2; Spr and Apr | This study |
| E. coli TG1 | Host strain for DNA clone | Stratagene |
| E. coli BL21(DE3) | Host strain for protein expression | Stratagene |
| E. coli ET12567(pUZ8002) | dam dcm strain containing helper plasmid pUZ8002 | 44 |
| Plasmids | ||
| pTA2 | TA clone vector; Amr | Toyobo |
| pET28a | Protein expression vector; Kmr | Novagen |
| pKJE7 | Plasmid with para-DnaK-DnaJ-GrpE | Takara |
| pIJ773 | Plasmid with FRT-aac(3)IV-FRT for disruption cassette amplification | 44 |
| pIJ779 | Plasmid with FRT-aadA-FRT for disruption cassette amplification | 44 |
| pSOK804 | Streptomyces-E. coli shuttle vector; Apr | 48 |
| pSOK804H | Streptomyces-E. coli shuttle vector with 6×His tag derived from pSOK804; Apr | This study |
| plip1 | Derived from pSOK804H carrying lip1 with native promoter and terminator | This study |
| plip2 | Derived from pSOK804H carrying lip2 with native promoter and terminator | This study |
| Fosmid SAF16H7 | Fosmid pCC2FOS carrying lip1; Cmr | This study |
| Fosmid SAF5A3 | Fosmid pCC2FOS carrying lip2; Cmr | This study |
| pET-lip1 | lip1 cloned into pET28a; Kmr | This study |
| pET-lip2 | lip2 cloned into pET28a; Kmr | This study |
a
Amr, ampicillin resistance; Apr, apramycin resistance; Spr, spectinomycin resistance; Kmr, kanamycin resistance; Cmr, chloramphenicol resistance; FRT, Flp recombination target.