TABLE 1.

Summary of purification processes of recombinant HMFOs^a

HMFO	Purification step	Total protein (mg)	Total activity (U)b	Sp act (U/mg)	Degree of purification (fold)	Yield (%)
MetspHMFO	Crude extract	1,660	284	0.17	1.0	100
	ResourceQ	71	187	2.62	15.4	66
	MonoQ	34	120	3.57	21.0	42
MetspHMFOHisc	Crude extract	1,910	100	0.05	1.0	100
	HiTrap	31	81	2.65	50.4	81
	MonoQ	17	56	3.30	62.9	56
PsespHMFO	Crude extract	1,720	138	0.08	1.0	100
	ResourceQ	50	71	1.42	17.8	52
	MonoQ	10	20	2.02	25.2	15
PseniHMFOHisc	Crude extract	1,650	12	0.01	1.0	100
	HiTrap	103	10	0.09	12.6	79
	MonoQ	47	8	0.16	22.2	63

^aData are for protein from 1 liter of E. coli culture.

^bMeasured with vanillyl alcohol in 50 mM Tris-HCl, pH 7.0, at 25°C.

^cHis-tagged protein.