TABLE 3.

Steady-state kinetic constants for the oxidation of vanillyl alcohol, HMF, DFF, and FDCA by HMFOs^a

^aMeasured at 25°C in 50 mM Tris-HCl, pH 7.0, except for vanillyl alcohol oxidation by PsespHMFO and PseniHMFO^His, which were measured at optimal pHs of 8 and 10, respectively. Units of measure are as follows: k_cat, min⁻¹; K_m, mM; k_cat/K_m, mM⁻¹ min⁻¹.

^bData fitted to Hill equation (n = 2.4 ± 0.5).

^cDue to substrate inhibition, MetspHMFO and MetspHMFO^His were not saturated and only a k_obs/[FFCA] value was obtained.