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Catalytic performance parameters for the production of FDCA from HMF by HMFOs in Tris-HCla
| HMFO | Optimal pH | Half-life (h) | TTNb | % FDCA production (mean ± SD) |
|---|---|---|---|---|
| MetspHMFOHis | 7.0 | 61 | 802 | 89 ± 4 |
| PsespHMFO | 7.5 | 40 | 584 | 65 ± 9 |
| PseniHMFOHis | 8.0 | 150 | 891 | 99 + 7 |
Production of FDCA from HMF at 1.5 mM by HMFOs at 5 μM in 50 mM Tris-HCl at 28°C after 96 h of reaction.
TTN, total turnover number.
