FIG 6.

Effects of polysaccharides and Com regulation system on biofilm formation. (A to C) A biofilm formation assay was performed in TSBg without bacitracin (A), with 0.0625 U/ml of bacitracin (1/2× MIC for the mbr mutants) (B), and with 0.5 U/ml of bacitracin (C). To assess the involvement of polysaccharides and Com-dependent quorum sensing in biofilm formation, we examined the 5-h biofilm formed by the polysaccharide synthase and com gene mutants. The data are the mean ± SD of three independent experiments. The data were analyzed with one-way ANOVA and a Bonferroni’s posttest. The asterisks indicate a significant difference (**, P < 0.01).