TABLE 4.
Primers for mutant construction
| Primer | Sequencea |
|---|---|
| mbrC uF | cccccgaattcCTTCCTGCCTTTACTTATGGC |
| mbrC uR | cccccggtaccTGGGCATGGTCATGCTCTTTC |
| mbrC dF | ccccctctagaAAGAGACTATCCAATTGACTCC |
| mbrC dR | cccccaagcttTGTCAGACTGTTCCTGCAGC |
| mbrD uF | cccccgaattcGTGCTAACGAGTTCACTAAGC |
| mbrD uR | cccccggtaccCCGCCAGTATAAAATGAGAC |
| mbrD dF | ccccctctagaACTGCTGCAGGAACAGTCTGAC |
| mbrD dR | cccccaagcttCTTAATGGTGATCTGCCCAC |
| brpA uF | GTTGGTACTCTCATTGGTACAGGAG |
| brpA uR | cccatgcgtttgggcccCTCCTAAAGCTACCAAGC |
| brpA dF | cccaaggagatggccggCAGATTACAGTAGCAGCG |
| brpA dR | CCATGGCTATCATCTCCAAGTGCTC |
| ermR F | gggcccaaacgcatgggAATACGCAAACCGCCTCT |
| ermR R | ccggccatctccttgggGTGCCACCTGACGTCTAA |
| 832 uF | GGCAAGGGCTATACAAAA |
| 832 uR-Bam | ccggatccGGCGGTAATGATTCTTAATAAAA |
| 832 dF-Bam | ccggatccCGTTGATTATTTATACGG |
| 832 dR | AGCGTCCATAACCGTAAT |
| 833 uF | AGAATTATTTTTGTAACCGCAGA |
| 833 uR-Bam | ccggatccTGGTTTCTTCCTCATTATAACAA |
| 833 dF-Bam | ccggatccGGAAACCAAGAAAAGACCA |
| 833 dR | GTCGTACTAAATTATTGCCGAA |
a
Additional sequences which do not correspond to the sequences of the template DNA are indicated by lowercase letters.