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. 2020 Aug 4;13:7759–7771. doi: 10.2147/OTT.S244699

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© 2020 Cheng et al.

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The expression of circLPAR3 was increased in esophageal squamous cell carcinoma (ESCC) tissues and cells. (A) The expression of circLPAR3 in ESCC tissues (ESCC) and adjacent normal tissues (Normal) was detected by qRT-PCR. (B) The expression of circLPAR3 in the peripheral blood of ESCC patients and healthy peoples was measured by qRT-PCR. (C) QRT-PCR was used to determine the circLPAR3 expression in ESCC cell lines (EC109, EC9706, KYSE30 and KYSE150) and HET-1A cells. (D) Kaplan-Meier analysis was performed to examine the correlation between circLPAR3 expression and overall survival of ESCC patients. (E) The relative expression levels of circLPAR3 and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in KYSE30 and KYSE150 cells were assessed by qRT-PCR after treatment with RNase R. (F) The expression levels of circLPAR3, U6 and GAPDH in the nuclear and cytoplasmic of KYSE30 and KYSE150 cells were detected by qRT-PCR after nuclear-cytoplasmic separation. *P < 0.05.