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. 2020 Aug 4;13:7759–7771. doi: 10.2147/OTT.S244699

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© 2020 Cheng et al.

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Effects of miR-375 and miR-433 inhibitors on esophageal squamous cell carcinoma (ESCC) progression. (A) The expression levels of miR-375 and miR-433 were detected by qRT-PCR to evaluate the transfection efficiency of anti-miR-375 and anti-miR-433. KYSE30 and KYSE150 cells were co-transfected with sh-circLPAR3#1 and anti-miR-375 or anti-miR-433. (B) The viability of KYSE30 and KYSE150 cells was measured by CCK-8 assay. (C) Colony formation assay was used to assess the number of colonies in KYSE30 and KYSE150 cells. (D, E) Transwell assay was performed to determine the number of migrated and invaded KYSE30 and KYSE150 cells. (F, G) The protein levels of proliferating cell nuclear antigen (PCNA), Ki-67, matrix metalloproteinase2 (MMP2) and MMP9 in KYSE30 and KYSE150 cells were tested by Western blot (WB) analysis. *P < 0.05.