Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 4;13:7759–7771. doi: 10.2147/OTT.S244699

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Cheng et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Effect of HMGB1 overexpression on esophageal squamous cell carcinoma (ESCC) progression. (A) The protein level of high-mobility group box 1 (HMGB1) was detected by Western blot (WB) analysis to evaluate the transfection efficiency of HMGB1 overexpression plasmid. KYSE30 and KYSE150 cells were co-transfected with (short hairpin) sh-circLPAR3#1 and HMGB1 overexpression plasmid. (B) CCK-8 assay was performed to measure the viability of KYSE30 and KYSE150 cells. (C) Colony formation assay was used to test the number of colonies in KYSE30 and KYSE150 cells. (D, E) The number of migrated and invaded KYSE30 and KYSE150 cells was assessed by transwell assay. (F) The protein levels of proliferating cell nuclear antigen (PCNA), Ki-67, matrix metalloproteinase2 (MMP2) and MMP9 in KYSE30 and KYSE150 cells were determined by WB analysis. *P < 0.05.