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. 2020 Aug 8;8:129. doi: 10.1186/s40478-020-00989-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Immunohistochemical analysis for 5-Lox and FLAP expression in human AD brain. a 5-Lox immunoreactivity (using monoclonal 5-Lox antibody from BD Biosciences #610694) in human post-mortem AD (high, Braak 6) hippocampus specimen. 5-Lox staining was prominent in cells of the granular cell layer in the dentate gyrus. b FLAP was expressed in cells with glial cell morphology but not in neurons. c Double staining of 5-Lox with FLAP revealed only minor co-expression of 5-Lox in FLAP positive cells (arrows). Asterisks indicate double positive cells. d Double staining of 5-Lox with microglia marker Iba1 showed that non-neuronal 5-Lox expression is associated to some microglia cells (asterisk). Arrows indicate microglia without 5-Lox expression. e Percentage of Iba1⁺/5-Lox⁺ and Iba1⁺/5-Lox⁻ cells in Iba1⁺ cells from control and AD (f) hippocampus sections. Hematoxylin (a, b) was used as nucleus stain. Scale: 20 μm (a-c, d right image), 50 μm (d, left image)