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3D Render of images with Imaris Software. 5-Lox immunoreactivity (red, using polyclonal 5-Lox antibody from Abcam #ab39347) is shown in NeuN positive neurons (green) of the granular cell layer in the dentate gyrus and in some Iba1 positive microglia cells (white). In microglia cells 5-Lox staining clustered at the nuclear membrane indicating an activated form of the 5-Lox enzyme (arrow). Scale: 20 μm and 5 μm inserts
