Table 1.

Tested 5-Lox and FLAP antibodies on mouse brain tissue and their immunoreactivity in neurons and/or microglia cells. aa = amino acid; − = no, + = mild, ++ = moderate and +++ = strong immunoreactivity

Antibody (host species)	Dilution	Reactivity	Epitope/antigen	Neurons	Microglia	Others
polyclonal Abcam #ab39347 (rabbit)	1:100	mouse, rat, hamster, human, pig	human 5-Lox aa. 130–149	++	++	+
monoclonal Abcam #ab169755 (rabbit)	1:100	mouse, rat, human	human 5-Lox aa. 100–200	++	+	–
monoclonal BD Biosciences #610694 (mouse)	1:50	chicken, human, rat, mouse	human 5-Lox aa. 442–590	+++	–	–
polyclonal Novus Biologicals NBP1–84666 (rabbit)	1:100	human, rat	recombinant protein	–	+++	–
polyclonal Novus Biologicals NB300–891 (goat)	1:150	human, mouse, rat	peptide corresponding to human FLAP (C-terminus)	–	++	–