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Primers used for real-time quantitative PCR
| Target gene | Direction | Sequence (5’-3’) |
|---|---|---|
| GAPDH | Forward | GGAGCGAGATCCCTCCAAAAT |
| Reverse | GGCTGTTGTCATACTTCTCATGG | |
| TNF-α | Forward | CGAGTCTGGGCAGGTCTA |
| Reverse | GTGGTGGTCTTGTTGCTTAA | |
| IL-1β | Forward | CCCTCTGTCATTCGCTCCC |
| Reverse | CACTGCTACTTCTTGCCCCC |
