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. 2020 Jun 29;12(4):plaa032. doi: 10.1093/aobpla/plaa032

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© The Author(s) 2020. Published by Oxford University Press on behalf of the Annals of Botany Company.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Multichannel imaging by CLSM. Hand-cut longitudinal (A) and transverse (B) sections of a root of Rhizophora apiculata visualized after double staining with fluorol yellow/Congo red. Single optical sections by CLSM (Zeiss LSM 880, Axio Imager 2, Objective lens: Plan-Apochromat 10/0.45 M27). Merged three channels (ex/em of 405/BP 415–498; 488/BP 543–579; 561/BP 590–630) showing blue for lignin, green for suberized cell walls and red staining of polysaccharides. (C) Maximum image projection of Tricoderma reesei hypha (false colour red) with oxidized lignin-bearing softwood fibres (green). Red hypha (calcofluor) collected with ex/em 405/424–502, green emission from FM 5-95 (fibre) ex/em 488/499–591 (Zeiss LSM 710). The FM 5-95 dye adsorbed on oxidized lignin producing much stronger signal than in membranes. Lignin autofluorescence has insignificant contribution. Bars = 100 µm.