Figure 6.

Effects of BYHWD on H₂O₂-induced cell apoptosis in HUVECs. Cells were double-stained with cell membrane-permeable (Hoechst 33342; blue) and impermeable (PI; red) DNA labeling fluorochromes. (a) Vehicle medium (control) for 6 h. (b) Preincubation with BYHWD (30 mg/ml) for 6 h. (c) H₂O₂ (400 μM) for 6 h. (d) Preincubation with BYHWD (10 mg/ml) for 6 h followed by H₂O₂ for 6 h. (e) Preincubation with BYHWD (20 mg/ml) for 6 h followed by H₂O₂ for 6 h. (f) Preincubation with BYHWD (30 mg/ml) for 6 h followed by H₂O₂ for 6 h. (g) The percentage of apoptotic cells for each group and time point. ^#P < 0.05 and ^##P < 0.01 versus the H₂O₂ group at the same time. Scale bars, 50 μm.