Figure 1.

The ablation of p75^NTR rescues acute amyloid-β induced changes in dendritic spine morphology. Primary hippocampal neurons from wild type (A, B–D) and p75^NTR ko (A, E–G) mice were incubated with vehicle (PBS; 0) or with 10, 50, 100, or 500 nM Aβ_1–42 oligomers for 6 h. (A) shows representative images as maximum intensity projections of dendrites of f-GFP⁺ expressing neurons, which were co-stained with MAP2 to visualize the entire dendritic network. An evaluation of the spine density (B, E), spine length (C, F), and spine head width (D, G) is shown in the graphs where dendritic segments with a length of ≥ 100 µm were used for analysis for wild type and p75^NTR ko neurons. Columns represent mean values + SEM and dots mark data points of individual neurons. The number of evaluated cells, obtained from three independent sets of experiments, is given in each column of the graphs. Statistical significance was tested using a one-way ANOVA with Sidak post-test. ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001. Scale bar: 20 µm.