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. 2020 Aug 7;10:13375. doi: 10.1038/s41598-020-70212-w

Figure 3.

Figure 3

Analysis of electrophysiological properties of HEK293 cells in KCNQ2 homomeric and heteromeric S247X channels. (A) (a) Representative current traces of KCNQ2 WT (2 μg) (n = 20), S247X (2 μg) (n = 10), and KCNQ2 WT + S247X (1 μg:1 μg) (n = 10). (b) Current density versus membrane potential (from − 80 to 40 mV) for KCNQ2 WT, S247X, and KCNQ2 WT + S247X. The current density demonstrated the lowest conductance–current curve in homomeric S247X for each membranous potential (− 80 to 40 mV), but the curve corresponds more closely with the KCNQ2 WT cells after the addition of KCNQ2. *p < 0.05 for KCNQ2 WT versus S247X. (c) Normalized currents (G/Gmax) versus voltage (from − 80 to 40 mV) for KCNQ2 WT (2 μg), S247X (2 μg), and KCNQ2 WT + S247X (1 μg:1 μg). The cells transfected with homomeric S247X exhibited lower normalized currents (from − 30 to 10 mV) than did cells with KCNQ2 WT. *p < 0.05 for KCNQ2 WT versus S247X. (B) (a) Representative current traces of KCNQ2 WT + KCNQ3 WT (1 μg + 1 μg) (n = 10) and KCNQ2 WT + S247X + KCNQ3 (0.5 μg + 0.5 μg + 1 μg) (n = 10). (b) Current density versus membrane potential (from − 80 to 40 mV) shows that the conductance–current curve in KCNQ2 WT + S247X + KCNQ3 corresponds closely to the curve of the KCNQ2 WT + KCNQ3 WT cells. (c) Normalized current versus voltage for two conditions of KCNQ2 WT + KCNQ3 WT (1 μg + 1 μg) and KCNQ2 WT + S247X + KCNQ3 (0.5 μg + 0.5 μg + 1 μg). Currents in the heteromeric transfected S247X cells were approximately equal to those in the KCNQ2 WT cells.